Human Leucyl/Cystinyl Aminopeptidase (LNPEP) ELISA Kit from MyBioSource.com

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Human Leucyl/Cystinyl Aminopeptidase (LNPEP) ELISA Kit

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Description

Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to LNPEP. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to LNPEP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain LNPEP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of LNPEP in the samples is then determined by comparing the O.D. of the samples to the standard curve